Journal: American Journal of Translational Research
Article Title: Senolytic controls bone marrow mesenchymal stem cells fate improving bone formation
doi:
Figure Lengend Snippet: Quercetin cleaned senescent BMSCs improving the proliferation of BMSCs. A. Representative microscopic images of the β-gal staining of the BMSCs in the P1 group, P15 group, P15+Vel group, and P15+Q group (magnification: 400×). B. Quantitative analysis of the percentage of β-gal positive BMSCs in the P1 group, P15 group, P15+Vel group, and P15+Q group. Values are shown as mean ± SD. NS, no significance, ****P<0.0001, one-way ANOVA with Tukey’s multiple comparisons test. C. CCK8 assay of the BMSCs in the P1 group, P15 group, P15+Vel group, and P15+Q group. Values are shown as mean ± SD. NS, no significance, ***P<0.001, one-way ANOVA with Tukey’s multiple comparisons test. D. Representative macroscopic photos of colony formation assay of the P1 group, P15 group, P15+Vel group, and P15+Q group (magnification: 10×). E. Quantitative analysis of colony formation assay of the P1 group, P15 group, P15+Vel group, and P15+Q group. Values are shown as mean ± SD. NS, no significance, **P<0.01, one-way ANOVA with Tukey’s multiple comparisons test.
Article Snippet: Cell counting kit 8 (CCK8) assay Cellular proliferation was evaluated using the CCK-8 (C0038, Dojindo, Japan) according to the manufacturer’s instructions.
Techniques: Staining, CCK-8 Assay, Colony Assay